olymerase hain eaction - A process carried out in a test tube that produces millions of copies of small sections of DNA. Using a heat resistant enzyme (DNA polymerase) and a mixture of other chemicals, cycles of hot and cold temperatures essentially photocopy a particular marker (or locus) of the DNA many times. Fluorescent tags are added to each copy so that they may be detected using laser analyzers. A technique called multiplexing enhances the process.
A method of producing relatively large amounts of specific regions of DNA, thereby making possible various analyses that are based on those regions.
olymerase hain eaction [DNA fragments synthesis].
A technique for copying the complementary strands of a target DNA molecule simultaneously for a series of cycles until the desired amount is obtained. First, primers are synthesized that have nucleotide sequences complementary to the DNA that flanks the target region. The DNA is heated to separate the complementary strands and then cooled to let the primers bind to the flanking sequences. A heat-stable DNA polymerase is added, and the reaction is allowed to proceed for a series of replication cycles. Twenty will yield a millionfold amplification; 30 cycles will yield an amplification factor of 1 billion.
A laboratory technique that permits a small DNA section located between two fixed points on the DNA molecule to be duplicated many times, yielding many copies of that DNA section.
Process involving isolation and amplification of a specific DNA sequence which can then be studied.
olymerase hain eaction. An amazingly sensitive technique that allows the specific amplification of extremely small amounts of particular DNA fragments using DNA polymerase and specific primers.
A technological method of amplifying a strand of DNA by many thousands through cycles of separation of DNA strands by raising the temperature, attachment of a pre-prepared added primer through the cooling of the temperature, and replication of strands using Taq polymerase. The cycle is repeated until a desired quantity of DNA is produced (Ford, 1997).
olymerase hain eaction - discovered in 1985, has revolutionized the DNA typing methods used in forensic casework.
A technique commonly used in DNA testing and research to analyze minute quantities of genomic DNA by amplification (making millions of copies) of a specific gene or region.
Polymerase chain reaction. A method for exponentially increasing the number of copies of a specific DNA sequence. The use of PCR enables the genetic analysis of biological samples containing only tiny amounts of DNA.
Polymerase chain reaction, a technique for copying the complementary strands of a target DNA molecule simultaneously for a series of cycles until the desired amount is obtained. First, primers are synthesized that have nucleotide sequences complementary to the DNA that flanks the target region. The DNA is heated to separate the complementary strands and the cooled to let the primers bind to the flanking sequences. A heat-stable DNA polymerase, Taq and excess deoxynucleotides are added, and the reaction is allowed to proceed for a series of replication cycles. An animation of PCR can be viewed at http://vector.cshl.org/Shockwave/pcranwhole.html.
The DNA polymerase chain reaction, a technique that allows small quantities of DNA to be selectively and repeatedly copied in a test tube. It can be used as a detection technique (detecting specific DNA sequences) or used to obtain sufficient quantities of DNA for further analysis. The PCR process is covered by patents owned by Hoffman-LaRoche Inc., and its use is limited by those patents and by licensing arrangements determined by the Perkin-Elmer Corporation.
Polymerase Chain Reaction. A technique invented in 1983 to make multiply small samples of DNA.
Polymerase chain reaction. An in vitro process for making many copies of a chosen fragment of DNA.
polymerase chain reaction. A type of nucleic acid amplification test.
Polymerase chain reaction. The CDC genotyping program uses two PCR-based techniques --- spoligotyping and MIRU analysis. Only a small amount of culture is needed for PCR-based genotyping, and the PCR test can be completed in 1day (because the PCR tests are batched, the actual turn-around time from receipt of a specimen to reporting the results can be longer).
polymerase chain reaction. A method used to amplify a DNA sequence by replicating it selectively and repeatedly from a DNA mixture.
polymerase chain reaction, a molecular technique used to amplify nucleotide sequences
polymerase chain reaction (a laboratory method for amplifying DNA or RNA of an organism to aid identification)
polymerase chain reaction. PCR is a technique for creating millions of copies of a specific region of DNA. Two short DNA "primers" adhere to sites flanking the DNA segment of interest. DNA polymerase added to the reaction mixture amplifies the DNA between the primers.
polymerase chain reaction. system for in vitro amplification of DNA that involves separating the DNA into its two complementary strands and using DNA enzymes to synthesize two-stranded DNA from each single strand, and repeating the process
Polymerase Chain Reaction. A technique allowing the production of multiple copies of extremely small amounts of DNA fragments using DNA polymerase and specific primers.
Polymerase chain reaction. A highly sophisticated technique during which a known sequence of DNA is copied rapidly over a short period, such as millions of copies over a few hours. PCR testing assists in diagnosing certain genetic disorders, helps identify individuals through analysis of a single cell or so-called "DNA fingerprinting," or characterizes certain strains of infectious microorganisms.
Polymerase chain reaction. Experimental technique used to amplify DNA.
Polymerase chain reaction. This is an in vitro technique for the selective amplification of defined nucleic acid regions in a DNA mixture by copying the complementary strands of a target DNA molecule simultaneously for a series of cycles until the desired amount is obtained. The principle: Primers are synthesised which have nucleotide sequences complementary to the DNA that flanks the target region. Then, the DNA is heated to separate the complementary DNA strands and cooled to allow the primers to bind to the flanking sequences. Heat-stable DNA (Taq) is added and the reaction allowed to proceed for a series of replication cyles. Twenty cycles will render an approximately millionfold amplification of the soruce DNA.
Polymerase chain reaction. A method for amplifying a DNA base sequence using a heat-stable polymerase and two primers, one complementary to the (+)-strand at one end of the sequence to be amplified and the other complementary to the (-)-strand at the other end. The faithfulness of reproduction of the sequence is related to the fidelity of the polymerase. Errors may be introduced into the sequence using this method of amplification.
Polymerase Chain Reaction. A testing technique that can identify the DNA or RNA (ie, the primary genetic material) of a specific organism. This type of test can identify hepatitis C virus RNA in a blood sample, and is the most specific test for hepatitis C infection.
Polymerase Chain Reaction. A method of DNA analysis that amplifies a specific DNA (gene) region allowing rapid DNA analysis.
Polymerase Chain Reaction, a process that examines a single strand of DNA to make prenatal diagnoses of genetic diseases or provide identification of an individual
Polymerase Chain Reaction. Technique which amplifies specific segments of DNA. -- PCR overview by Access Excellence is a good reference
Polymerase chain reaction. A technique for copying and amplifying the complementary strands of a target DNA molecule. It is an in vitro method that greatly amplifies, or makes millions of copies of, DNA sequences that otherwise could not be detected or studied.
Polymerase chain reaction. A technique used to create a large number of copies of a target DNA sequence of interest. One use of PCR is in the detection of DNA sequences that indicate the presence of a particular genetically engineered organism.
Polymerase Chain Reaction. Method of copying short DNA sequences millions of times in vitro.
polymerase chain reaction; a technique used to amplify a gene of interest.
Polymerase Chain Reaction. A process used in DNA identification testing in which one or more specific small regions of the DNA are copied using a DNA polymerase enzyme so that a sufficient amount of DNA is generated for analysis.
a sensitive laboratory technique used to detect the amount of HIV RNA in the blood. PCR is used to measure viral load in persons infected with HIV.
Polymerase chain reaction. A techique for the cyclic amplification of DNA segments by thermostable DNA polymerase. Following heat denaturation of the DNA, oligonucleotide primers complementary to flanking regions are annealed and extended, resulting in the synthesis of replicate
polymerase chain reaction. A method of amplifying or copying DNA fragments that is faster than cloning. The fragments are combined with DNA polymerase, nucleotides, and other components to form a mixture in which the DNA is cyclically amplified.
A process allowing the multiplication and detection of minute traces of DNA.
polymerase chain reaction. a procedure where a piece of DNA is copied many times to do genetic analysis. It can be used to amplify very small amounts of DNA.
Polymerase chain reaction. PCR is a technique used to make additional copies of DNA from a small sample of DNA. It is a very reliable way to tell if someone has HSV infection, but is available in Canada only through special laboratories. A sample for testing using PCR should be taken from a sore; a negative PCR result from a swab taken from healthy skin does not rule out HSV.
a systematic, primer mediated enzymatic process for the geometrical amplification of a target DNA sequence. PCR product can be generated from as little as one molecule of target material (DNA or RNA) under optimal conditions.
see polymerase chain reaction.
Polymerase Chain Reaction. The process of target amplification used in the AMPLICOR® Microwell Plate Tests and COBAS AMPLICOR Analyzer Automated PCR Tests.
This has two meanings for CNTech. It stands for CNTech’s Process Cleanroom. It also is the abbreviation for polymerase chain reaction, a molecular biological technique commonly used to amplify DNA.
Polymerase chain reaction. An efficient, simple, and rapid technique to amplify a segment of DNA in a test tube (see text of notes for a more complete explanation).
polymerase chain reaction. the first practical system for in vitro amplification of DNA, and as such one of the most important recent developments in molecular biology.
(Polymerase Chain Reaction): The amplification of DNA segments. An important step in the process of determining DNA marker values.
Polymerase chain reaction. A technique used to enzymatically amplify the number of copies of a sequence of DNA.
Polymerase chain reaction, a method of amplifying fragments of genetic material so that they can be detected. Some viral load tests use this method.
Polymerase Chain Reaction. A method for enzymatically and exponentially amplifying a selected region of DNA
polymerase chain reaction is a technique for amplifying a specific sequence in DNA by repeated cycles of synthesis driven by pairs of reciprocally oriented primers.
Polymerase chain reaction. a method using sequential DNA polymerase reactions to amplify a single copy of gene segment
Polymerase Chain Reaction. A technique used in genetic (DNA) diagnosis.
polymerase chain reaction. Multiplying a particular DNA segment in repeated cycles. The "copies" made in a previous cycle are used as "originals" or templates in the next cycle. For example, PCR enables forensics experts to do DNA testing on very small blood samples.
a process developed in mid-1980’s to make a large number of copies of a DNA sequence from very little DNA. It is used in forensics when little DNA is available for testing.
Polymerase Chain Reaction. A very sensitive test used in research to detect minute amounts of DNA from an organ.
a technique that amplifies nucleic acid sequences exponentially.
polymerase chain reaction. a technique for making many copies of a specific DNA sequence. The reaction is initiated using a pair of short primer sequences which match the ends of the sequence to be copied. Thereafter, each cycle of the reaction copies the sequence between the primers. Primers can bind to the copies as well as the original sequence, so the total number of copies increases exponentially with time.
Polymerase chain reaction. A laboratory method used to make many copies of a specific DNA sequence.
Polymerasel Chain Reaction. Utilizes oligonucleotide primers and a heat stable DNA polymerase to amplify a segment of DNA. Permits rapid genotyping of marker alleles from minute amounts of genomic DNA
Polymerase Chain Reaction: a molecular biological technique performed in the laboratory to manufacture additional DNA strands from small numbers of DNA strands in the original specimen.
Polymerase chain reaction. A technique for the rapid production of millions of copies of a particular stretch of DNA.
Polymerase chain reaction. A test performed to evaluate false-negative results to the ELISA and Western blot tests. In PCR testing, numerous copies of a gene are made by separating the two strands of DNA containing the gene segment, marking its location, using DNA polymerase to make a copy, and then continuously replicating the copies. The amplification of gene sequences that are associated with HIV allows for detection of the virus by this method.
(synonym: polymerase chain reaction) A procedure that produces millions of copies of a short segment of DNA through repeated cycles of: 1) denaturation, 2) annealing, and 3) elongation; PCR is a very common procedure in molecular genetic testing and may be used to: 1) generate a sufficient quantity of DNA to perform a test (e.g., sequence analysis, mutation scanning), or 2) may be a test in and of itself (e.g., allele-specific amplification, trinucleotide repeat quantification). Related Terms: Southern blot ; X-chromosome inactivation study ; conformation-sensitive gel electrophoresis ; denaturing gradient gel electrophoresis ; mutation analysis ; mutation scanning ; restriction fragment length polymorphism analysis ; sequence analysis ; single-stranded conformational polymorphism
Polymerase chain reaction, a sophisticated technique to amplify a small amount of DNA.
polymerase chain reaction. a powerful technique for synthesizing a large amount of DNA from very small amounts of DNA having a specific sequence. This is accomplished through repeated cycles of denaturation, primer anneali ng, and replication (extension) using a heat-resistant DNA polymerase. Is also used in DNA sequencing.
Polymerase Chain Reaction. A technique to expand trace amounts of DNA or RNA so that the specific type of the DNA or RNA can be studied or determined. This technique has become useful in detecting a very low concentration of residual leukemia or lymphoma cells, too few to be seen using a microscope. The technique can detect the presence of one leukemic cell among five hundred thousand to one million non-leukemic cells. PCR requires a specific DNA (or RNA) abnormality or marker, like an oncogene, in the leukemic or lymphomatous cells for its use to identify residual abnormal cells.
Polymerase Chain Reaction, a method to detect and multiply traces of certain DNA or RNA chains.
Polymerase chain reaction. A technique used to amplify or generate large amounts of replica DNA or a segment of any DNA whose ``flanking'' sequences are known. Oligonucleotide primers that bind these flanking sequences are used by an enzyme to copy the sequence in between the primers. Cycles of heat to break apart the DNA strands, cooling to allow the primers to bind, and heating again to allow the enzyme to copy the intervening sequence lead to doubling of the DNA present at each cycle.
Polymerase Chain Reaction. Automated enzymatic method for the specific and quick reproduction of DNA segments.
Polymerase chain reaction. An in vitro technique to produce many copies of a specific section of DNA sequence. PCR is normally used to amplify sections up to ~2kbp in length, although routine PCR of sections up to 20kbp is becoming possible. PCR amplification is possible from complex sequence background e.g. a short sequence from an entire chromosome, and from impure DNA. The technique is widely used in many applications.
POLYMERASE CHAIN REACTION. a highly sensitive test that uses an amplification technique to detect small amounts of DNA or RNA in blood or tissue samples.
POLYMERASE CHAIN REACTION. 1. A laboratory process that selects a DNA segment from a mixture of DNA chains and rapidly replicates it; used to create a large, readily analyzed sample of a piece of DNA. It is used in DNA fingerprinting and in medical tests to identify diseases from the infectious agent's DNA. (See DNA.) 2. As related to HIV -- also called RT-PCR -- a sensitive laboratory technique that can detect and quantify HIV in a person's blood or lymph nodes. PCR works by repeatedly copying genetic material using heat cycling and enzymes similar to those used by cells. It is an FDA-approved test to measure viral load.
A sensitive test that amplifies DNA. PCR is a critical part of tests for viral load, genotyping, and phenotyping.
Polymerase chain reaction. A molecular technique in which the DNA of plant or microbe is used to generate banding patterns that can be used in determining the relationship between organisms.
a sensitive laboratory technique used to detect and repeatedly copy small amounts of RNA or DNA. Some PCR tests can also quantify the amount of RNA or DNA. PCR is used to measure viral load in persons infected with HIV.
olymerase hain eaction; this is an advanced biochemical test used to identify the presence of specific pieces of an organisms genes (DNA)
Polymerase Chain Reaction. A chemical process that replicates a given sample of DNA many times, in imitation of natural replication. The process cycles between two stages: splitting the two strands of DNA apart and then forming new double strands by adding a mixture of the four DNA bases. By adding primers as well, the process can be used to replicate just the one or more DNA segments of interest.
Polymerase chain reaction. a common method of creating copies of specific fragments of DNA
Polymerase Chain Reaction - refers to a laboratory method used to test for the actual virus
Polymerase Chain Reaction is a process used to amplify pieces of the genetic make-up of a cell or virus. The amplified pieces are then detected and the presence of the virus itself can be determined.
polymerase chain reaction. A fast, inexpensive technique for making an unlimited number of copies of any piece of DNA. Sometimes called "molecular photocopying," PCR has had an immense impact on biology and medicine, especially genetic research.
Polymerase Chain Reaction. is a molecular biology technique for emzymatically amplifying (creating multiple copies of) DNA without using a living organism, such as E. coli or yeast. The technique allows a small sample of DNA to be copied multiple times so it can be used for analysis, for example to detect contamination with (known) GMOs. Unknown transgenic DNA cannot be detected by PCR. For details and a description of the procedure check Wikipedia
An enzyme mediated process that can yield millions of copies of a targeted DNA sequence. View Link
Polymerase Chain Reaction. A method for amplifying specific DNA sequences in a laboratory. Many millions of copies of the DNA can be produced in just a few hours. The details of how PCR occurs can be found in any good Biology textbook. We've put our version of a PCR diagram on another page.
Polymerase Chain Reaction. Français] A method that simulates an environment for rapid DNA replication of specific DNA segments. This procedure results in multiple copies of the specific DNA sequence which are then used in various experiments for diagnostic and analytical purposes.
The polymerase chain reaction is a technique for quickly "amplifying" a particular piece of DNA in the test tube (rather than in living cells). Thanks to this procedure, it's possible to make virtually unlimited copies of a single DNA molecule even though this molecule may be present in a mixture containing many other different DNA molecules. In order to perform PCR, you must know at least a portion of the sequence of the DNA molecule that you wish to replicate.
polymerase chain reaction. in vitro method for rapid amplification of DNA
Polymerase Chain Reaction. a method used to replicate specific portions of the DNA strands. The DNA is heated, causing the two strands to separate like a zipper. The two DNA halves are then cooled and mixed with a special enzyme. The result of this process is the creation of two DNA strands identical to each other and to the original DNA strand. This process is repeated many times to replicate a desired DNA sequence millions of times in a matter of hours. PCR is especially valuable because it does not require high quality or large quantities of DNA. Also, this method lends itself to automation and less labor-intensive typing. The PCR/STR analysis process includes five stages, which are extraction, quantification, amplification, electrophoresis, and data interpretation.
Polymerase Chain Reaction. A method of replicating very small amounts of DNA which can be used in HIV testing to detect HIV viral DNA prior to seroconversion of the individual. .
Polymerase Chain Reaction is an artificial replication system based on numerous replication cycles (30-40) where DNA is doubled constantly.
Polymerase chain reaction. A laboratory technique that amplifies the genetic material of a virus to a level that can be detected. The presence or absence of the virus can then be determined. PCR is used to directly measure for the hepatitis C infection.
See polymerase_chain reaction.
Polymerase chain reaction. A method for amplifying a DNA base sequence using a heat- stable polymerase and two 20- base primers, one complementary to the (+)- strand at one end of the sequence to be amplified and the other complementary to the (- )- strand at the other end. Because the newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
Polymerase Chain Reaction. a method to produce sufficient DNA for analysis from a very small amount of DNA.
Polymerase chain reaction. A method used to make multiple copies of DNA. A stage used in the examination of a DNA sample to determine whether they have a genetic mutation or not.
(Polymerase Chain Reaction) Process of exponentially amplifying DNA to generate sufficient sample amount for diagnostic analysis
A highly sophisticated scientific method of detecting the presence of hepatitis B virus DNA or hepatitis C virus RNA in the blood. This test can be conducted on the same sample of blood obtained with the hepatitis B panel of blood tests; no extra doctor’s visit is needed.
polymerase chain reaction. a method for creating millions of copies of a particular segment of DNA. If a scientist needs to detect the presence of a very small amount of a particular DNA sequence, PCR can be used to amplify the amount of that sequence until there are enough copies available to be detected.
a technique for replicating DNA or RNA in vast quantities ( 1,000,000 copies). It involves cycles of heating (denaturization) and cooling (annealing) of a nucleotide template, or primer, in the presence of random, single, nucleotides and special enzymes, which allow them to be incorporated into replicated fragments in the proper order. The copies can be used for paternity testing, screening for genetic diseases, and other scientific analysis.
Polymerase chain reaction. A serial reaction involving the use of a heat-stable DNA polymerase to amplify a DNA sequence millions of times in a few hours.
(Polymerase Chain Reaction) A sensitive laboratory method used to detect and measure amounts of RNA or DNA. Used to determine viral load in people infected with HIV.
Polymerase chain reaction. A very sensitive test used to detect very low levels of bcr-abl in marrow stem cells.
The polymerase chain reaction (PCR) is a laboratory technique used to make millions of copies of a known piece of DNA, used in many medical and forensic tests.
polymerase chain reaction. The process whereby a segment of DNA is copied or cloned, using DNA polymerase so that its sequence is multiplied many times in a laboratory.
Polymerase chain reaction. The fragments are then amplified by cloning or by polymerase chain reaction (PCR) methods. (IOOakRidge) Reação em Cadeia da Polimerase (PCR) K. Mullis desenvolve o método de PCR (Reação em Cadeia da Polimerase). (POPrGenoma)
Polymerase chain reaction. An enzyme-mediated technique that allows specific DNA sequences to be amplified.
Polymerase chain reaction. A very sensitive test for the presence of HIV.
Polymerase chain reaction. An amazingly sensitive technique that allows the specific amplification of extremely small amounts of particular DNA fragments using DNA polymerase and specific primers.
The Polymerase Chain Reaction, used to amplify DNA lying between two target sequences.
Polymerase chain reaction. A method for amplifying DNA in vitro, involving the use of oligonucleotide primers complementary to nucleotide sequences in a target gene and the copying of the target sequences by the action of DNA polymerase.
The polymerase chain reaction, a quick and easy method for generating unlimited copies of any fragment of DNA.
Polymerase chain reaction. A laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated, producing a large, readily analyzed sample of a piece of DNA.
A method of increasing the quantity of a specific DNA sequence.
Polymerase chain reaction. A "biological copy machine": a method for making many copies of a specific DNA base sequence.
polymerase chain reaction. A method for amplifying specific DNA segments that exploits certain features of DNA replication.
describes a technique in which cycles of denaturation, annealing with primer, and extension with DNA polymerase, are used to amplify the number of copies of a target DNA sequence by106 times.
Polymerase Chain Reaction. A technique used in the process of DNA profiling.
polymerase chain reaction; a process for amplifying specific DNA sequences in a test tube by repetitive cycles of DNA replication, usually conducted in a microprocessor-controlled automated machine
Polymerase chain reaction. a powerful technique for producing millions of copies of a specific region of DNA, so it can be analyzed as readily as can a purified chemical substance. PCR has been instrumental in major breakthroughs in diagnostic kit development, forensic medicine, and detection of genes associated with inborn errors of metabolism. A Nobel Prize in medicine was awarded in 1993 for the development of PCR.
Polymerase Chain Reaction. A method (developed by Kary Banks Mullis in 1983) for amplifying a DNA base sequence using a heat-stable polymerase and two 20-base primers, one complementary to the (+)-strand at one end of the sequence to be amplified and the other complementary to the (-)-strand at the other end. Because the newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. Several variations have been developed for specific needs. For example, PCR may be combined with reverse transcription of mRNA to cDNA to amplify an mRNA, forming so called RT-PCR.
polymerase chain reaction, a methodology used to produce multiple copies of selected segments of DNA molecules
Polymerase Chain Reaction. An amplification test for chlamydia. A process whereby a strand of DNA can be cloned (replicated) millions of times within a few hours.
Polymerase chain reaction. A laboratory method used to make many copies of a DNA fragment in minutes using an enzyme called polymerase.
Polymerase chain reaction. An laboratory technique for amplifying a large number of copies of a specific DNA segment flanked with two known target sequences called primers.
polymerase chain reaction, a technique that uses an enzyme (DNA polymerase) to repeatedly amplify specific regions of a DNA molecule, as a result of cycles of denaturation, polymerisation and elongation.
Acronym for Polymerase Chain Reaction. The method of amplifying specific regions of DNA by multiple cycles of DNA polymerization. Each cycle of polymerization is followed by a brief heat treatment to separate complementary strands.
The polymerase chain reaction. Amplification of DNA through cycles of denaturation (by heating) and synthesis of new strands. Facilitated by the availability of a heat-stable polymerase.
A method of detecting DNA or RNA from tissues or body fluids. PCR is used to determine if hepatitis B or hepatitis C virus is in the blood.
Polymerase chain reaction (also, NAT = nucleic acid testing)
Polymerase Chain Reaction. A method for creating a large number of copies of a specific piece of DNA. It uses a version of DNA polymerase that can function at high temperatures together with an automated temperature-controlled device known as a thermocycler. See the PCR section of the lab for details.
Polymerase chain reaction. One of several highly sensitive tests that measure the amount of viral genetic material (HCV RNA, for example) in the blood (see ‘viral load' and ‘bDNA assay').
The laboratory technique for duplicating (or replicating) DNA using the bacterium Thermus aquaticus, a heat stable bacterium from the hot springs of Yellowstone. As with the polymerase reaction that occurs in cells, there are three stages of a PCR process: separation of the DNA double helix, addition of the primer to the section of the DNA strand which is to be copies, and synthesis of the new DNA. Since PCR is run in a single reaction vessel, the reactor contains all of the components necessary for replication: the target DNA, nucleotides, the primer, and the bacterial DNA polymerase. PCR is initiated by heating the reaction vessel to 90° which causes the DNA chains to separate. The tempature is lowered to 55° to allow the primers to bind to the section of the DNA that they were designed to recognize. Replication is then initiated by heating the vessel to 75°. The process is repeated until the quantity of new DNA desired in obtained. Thirty cycles of PCR can produce over 1 million copies of a target DNA.
Otherwise known as Polymerase Chain Reaction. A technique used to amplify a specific DNA sequence. Many different PCR techniques are available.
Polymerase Chain Reaction. A laboratory process that selects a DNA segment from a mixture of DNA chains and rapidly multiplies it to create a large sample of a piece of DNA. It is a sensitive laboratory technique that can detect and measure HIV in a person's blood or lymph nodes (also called RT-PCR). It is also a means of measuring the amount of virus in the blood (viral load).
Polymerase chain reaction. A technique for making multiple copies of a specific stretch of DNA or RNA; can be used to test for mutations in DNA. For example, if a stretch of DNA is mutated, the copies of it made with the PCR can be longer or shorter than normal.
polymerase chain reaction. Molecular technique used to replicate and amplify a fragment of DNA
Polymerase chain reaction. a laboratory procedure used to amplify a specific segment of DNA by use of certain primers, enzymes and conditions. A minute amount of DNA is rapidly turned into a measurable quantity of the gene to be tested.
polymerase chain reaction. a method used to make multiple copies of DNA by a process developed in the mid-1980s. It is used to detect the existence of specific microbial genes in DNA samples extracted from infected blood, tissue or ticks.
Polymerase chain reaction. Amplification of specific lengths of DNA by repeated 'thermal cycling' reactions using polymerase enzyme.
polymerase chain reaction. Method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Polymerase Chain Reaction. A technique developed during the s that uses enzymes to copy short segments of DNA allowing a few starting molecules to be amplified to enormous quantities for sequencing or other research
A DNA synthesis system that takes advantage of a number of unique properties of DNA synthesis. Primers of a known sequence along with a DNA template, free nucleotides and buffers are added together. Repeated DNA melting and synthesis allows the gene sequence between the primer sequences to be repeatedly synthesized. After 30 cycles, billions of copies of that specific sequence are present in the reaction tubes. At the completion of a PCR reaction, the researcher needs to purify the PCR product away from the buffers, primers and building blocks. This can be done with a Nanosep® 100K centrifugal device.
Polymerase Chain Reaction. Polymerase Chain Reaction, or PCR, is a process which permits scientists to make unlimited copies of genes. This is done with a single molecule of DNA. One hundred billion copies of the DNA can be generated in a few hours. This technique is used to investigate and diagnose bacterial diseases, viruses associated with cancer, and genetic diseases such as diabetes mellitus.
Polymerase Chain Reaction. A reaction that uses the enzyme DNA polymerase to catalyze the formation of more DNA strands from an original one by the execution of repeated cycles of DNA synthesis. Procaryotes: Simple organisms that lack a distinct nuclear membrane and other organelles. See Eucaryotes.
Polymerase chain reaction; a technique using DNA polymerase to make multiple copies of a DNA template in vitro.
polymerase chain reaction. A method of amplifying low levels of specific DNA sequences in a sample, thus allowing one to detect very low levels of antigen or antibody in the sample.
Polymerase chain reaction. A technique which amplifies the number of copies of specific regions of DNA in order to produce enough DNA to be sufficiently tested. DNA is double-stranded (except in some viruses), and the two stands pair up in a very specific way. A gene's "building-block sequence" is the specific order of appearance of 4 different deoxyribonucleotides within a segment of DNA. These 4 components are: adenine (A), thymadine (T), cytosine (C), and guanine (G). The arrangement of this 4-letter alphabet generates a gene sequence. In this technique DNA is heated (denaturization) in order to separate the 2 strands. Primers are added and then the DNA is cooled in order to allow double-strands to form again. An enzyme is then added in cycles, which can "read" the gene sequence, and result in multiplication of DNA. PCR is utilized to diagnose gene-specific diseases, as well as disease-causing viruses and/or bacteria, or to link a criminal suspect to a crime.