A laboratory procedure that determines the order of base pairs in a segment of DNA.
Determining the order of nucleotides in a segment of DNA.
The experimental process of determining the nucleotide sequence of a region of DNA. This is done by labelling each nucleotide (A, C, G or T) with either a radioactive or fluorescent marker which identifies it. There are several methods of applying this technology, each with their advantages and disadvantages. For more information, refer to a current text book. High throughput laboratories frequently use automated sequencers, which are capable of rapidly reading large numbers of templates. Sometimes, the sequences may be generated more quickly than they can be characterised.
The determination of DNA base sequence order.
A procedure that determines the order of base pairs in DNA. Some genetic tests rely on sequencing to find areas of a gene that deviate from the norm (mutations) and may cause disease.
The process of determining the precise sequence of bases in a length of DNA.
The technique in which the specific sequence of bases forming a particular DNA region is deciphered.
a laboratory technique which is used to find out the sequence of nucleotide bases in a DNA molecule.
In the "plus and minus" or "primed synthesis" method, DNA is synthesized in vitro in such a way that it is radioactively labeled and the reaction terminates specifically at the position corresponding to a given base; in the "chemical" method, single stranded DNA is subjected to several chemical cleavage protocols that selectively make breaks on one side of a particular base.
The process of deciphering the precise order of nucleotide bases in a DNA molecule.
Laboratory procedure for determining the exact order of bases (ATCG) in a strand of DNA.In "bi-directional sequencing" the sequence of both complementary strands is obtained.
Français] The process of determining the exact order of the bases in a DNA segment.
The process of determining the exact order of the nucleotide bases in a segment of DNA.
Determining the pattern or order in which the nucleotide bases occur in a piece of DNA. This sequence is the genetic code.
method for deciphering the relative order of nucleotide bases in a DNA molecule
Decoding a strand of DNA or gene into the specific order of its nucleotides: adenine, cytosine, guanine and thymine. This analysis is done today manually or with automated equipment.
a laboratory technique used to determine the exact order of the base pairs in a segment of DNA.
The process by which scientists determine the exact order of the base pairs in a segment of DNA.
A technique for determining the exact order of nucleotides in a DNA molecule. This information is often useful in determining the likely function of that DNA molecule.
determining the order of nucleotides (adenine, thymine, guanine, and cytosine) in a section of DNA
Determination of the order of nucleotides (base sequences) in a DNA molecule.
But in contrast to DNA sequencing ... genome mapping still can only be accomplished by experienced scientists. (IOCeleraGenome) Seqüenciamento de DNA No caso de seqüenciamento de DNA, há um diferencial, que é o volume de informação. (POFapesp2)
Determination of the order of bases in a DNA molecule.
a technique for determining the order of nucleotide bases in a segment of DNA. This technique was made possible by the Nobel Prize-winning development of molecular cloning techniques for replicating genetic material. Automated sequencing technology is available now, and researchers are beginning to sequence entire genomes of free-living organisms, such as bacteria, yeast (single-celled fungi), and humans.
Determining the exact order of the base pairs in a segment of DNA using established scientific techniques.
Deciphering the order of bases within a piece of DNA.
The process by which the exact order of the base pairs in a segment of DNA is determined. Gene A segment of DNA that contains the information necessary to make a protein.
Analysis of the nucleotide sequence of a gene or DNA fragment.
Determining the exact order of the base pairs in a segment of DNA. [Talking Glossary
DNA sequencing is the process of determining the nucleotide order of a given DNA fragment, called the DNA sequence. For thirty years a huge fraction of DNA sequencing has been achieved using the chain termination method http://www.pubmedcentral.gov/articlerender.fcgi?artid=431765, developed by Frederick Sanger in 1975. This technique uses sequence-specific termination of an in vitro DNA synthesis reaction using modified nucleotide substrates.
