PCR using the enzyme reverse transcriptase (RT), a molecular technique used to amplify RNA sequence into DNA

Reverse Transcriptase Polymerase Chain Reaction: The mRNA is first reverse-transcribed into cDNA, and the cDNA is then amplified to measurable levels using PCR. PCR primers for all genes of interest are required.

Reverse transcriptase polymerase chain reaction: Powerful technique for producing millions of copies of specific parts of the genetic code of an organism so that it may be readily analyzed. More specifically, RT-PCR produces copies of a specific region of complementary DNA that has been converted from RNA. The technique is often used to help in the identification of an infectious agent.

see reverse transcriptase polymerase chain reaction test.

PCR amplification from an RNA template. The first step involves synthesis of a single strand of cDNA on the RNA template using reverse transcriptase. The cDNA is then used as the template for PCR to produce a DNA product.

(Reverse Transcriptase Polymerase Chain Reaction). An FDA-approved test to measure viral load. The test is also known as PCR (see Polymerase Chain Reaction).

reverse transcriptase - polymerase chain reaction. A technique commonly employed in molecular genetics through which it is possible to produce copies of DNA sequences rapidly. Qualitative RT-PCR for HCV test to detect HCV RNA by amplification of viral genetic sequences. Quantitative assays for HCV RNA tests to detect HCV RNA concentration (viral load) by amplification of viral genetic sequences or by signal amplification.

Reverse-transcription polymerase chain reaction