The formation of a phosphodiester bond between two adjacent bases separated by a single-strand break. Catalyzed by DNA ligase.
The joining of two linear nucleic acid molecules by the formation of phospho-diester bonds. In cloning experiments, a restriction fragment is often ligated to a linearized vector molecule using T4 DNA ligase.
Joining of DNA fragments to produce a single DNA molecule. Ligases are enzymes which perform this reaction. Fragments of plant DNA are ligated into bacterial plasmids during the cloning of probes for use for RFLP analysis.
The process of splicing two pieces of DNA together. In practice, a pool of DNA fragments are treated with ligase (see "Ligase") in the presence of ATP, and all possible splicing products are produced, including circularised forms and end-to-end ligation of 2, 3 or more pieces. Usually, only some of these products are useful, and the investigator must have some way of selecting the desirable ones.
To tie together or tie off. Examples include ligating a blood vessel to stop it bleeding, or ligating fragments of DNA together enzymatically.
In vitro reaction used to make the covalent phosphodiester linkage between two double stranded DNA ends. The reaction requires the presence of a phosphate group on the free 5' end and a -OH group on the free 3' end of the strands to be joined. The reaction is catalysed by the enzyme DNA ligase.
Joining two fragments of DNA end to end. See sticky ends.
Formation of a phosphodiester bond to link two adjacent bases separated by a nick in Pdouble-stranded DNA.