an enzyme that connects the Okazaki fragments (short, single-stranded DNA fragments) during DNA replication, producing a complementary strand of DNA.
An enzyme that closes nicks or discontinuities in one strand of double-stranded DNA by creating an ester bond between adjacent 3' OH and 5' PO4 ends on the same strand.
Enzyme involved in the replication and repair of DNA molecules. Ligase seals nicks in the backbone of one strand of a double stranded DNA molecule. It catalyses the formation of a (phosphodiester) bond between the 5' and 3' ends of the nicked DNA backbone.
In recombinant DNA technology, an enzyme that seals together two DNA fragments from different sources to form a recombinant DNA molecule.
An enzyme that can join nucleotides in a DNA strand.
An enzyme which can join two pieces of complimentary DNA together.
Enzymatic activity responsible for creating phosphodiester bonds between the 5? end of one strand of DNA and the 3? end of another strand. Requires the presence of a 5? phosphate on one strand, and a 3? hydroxyl group on the second strand.
the enzyme that bonds the Okazaki fragments together.
An enzyme that catalyses the reaction which links together two DNA molecules - used in linking DNA fragments together at their sticky ends formed previously with a restriction enzyme.
An enzyme capable of repairing breaks in the DNA backbone by forming the phosphodiesterbond that is placed between the 5' phosphate and the 3'OH group of ribose.
An enzyme able to form a phosphodiester bond between adjacent but unlinked nucleotides in a double helix.
a DNA replication enzyme that catalyzes the formation of covalent bonds between the 3' end of a new DNA fragment and the 5' end of the growing strand.
An enzyme which catalyses the formation of a phosphodiester bond between a 3'-hydroxyl and a 5'-phosphate group in DNA thereby joining two DNA fragments.
In molecular biology, DNA ligase is a particular type of ligase that can link together DNA strands that have double-strand breaks (a break in both complementary strands of DNA). The alternative, a single-strand break, is easily fixed by DNA polymerase using the complementary strand as a template but still requires DNA ligase to create the final phosphodiester bond to fully repair the DNA.
