Conditions affecting the hybridization of nucleotide sequences. Higher stringency conditions require more base pairing between the two sequences. Higher stringency conditions can be obtained by higher temperatures, highter salt concentrations, or addition of formamide.
Strictness, closeness, rigor.
A measure of the rigorousness of an attempt to separate two components. This term is often applied to nucleic acid hybridization conditions to indicate the likely degree of sequence homology between probe and target sequences.
the set of conditions under which hybridization of a nucleic acid probe with its complementary target sequence occurs. These conditions include the concentration of salt and formamide, temperature, length, and GC content of the probe, and percentage of mismatch of the probe with its target. These variables can be adjusted to either increase or decrease stringency, and thus the specificity of the hybridization reaction.
Reaction conditions- temperature, salt, pH. Dictate annealing of single-stranded DNA/DNA, DNA/RNA, and RNA/RNA hybrids. At high stringency, duplexes form only between strands with perfect one-to-one complementarity; lower stringency allows annealing between strands with some degree of mismatch
A parameter that lets you filter the results of a LifeSeq query based on how closely related the sequences in a gene bin must be.