(Tm) The temperature at which the two strands of a double-stranded nucleic acid molecule or base-paired hybrid detach due to dissolution of the hydrogen bonds that hold the pairs together.
(Tm) The temperature at which a double-stranded DNA or DNA-RNA molecule denatures.
The melting temperature refers to the midpoint in the transition from one state to another; for example, from folded to unfolded, or, in the case or a powder, from rigid to viscous.
(Tm) - the temperature point in a hybridization reaction at which 50% of the nucleotides are annealed (double strands) and 50% are denatured (single strands). The melting temperature is dependent on a set of conditions which are referred to as stringency.
The temperature at which one half of a particular DNA duplex will dissociate and become single strand DNA.
is the midpoint of the temperature at which 1/2 of the DNA molecules are denatured and 1/2 are annealed.
The dissociation of a double-stranded DNA molecule is often referred to as melting because it occurs quickly once a certain temperature has been reached. For multiple copies of a specific DNA molecule (usually synthesized by polymerase chain reaction), the melting temperature (Tm) is defined as the temperature at which 50% of that same DNA molecule species form a stable double helix and the other 50% have been separated to single strand molecules. The melting temperature depends on both the length of the molecule, and the specific nucleotide sequence composition of that molecule.
