electrophoresis to separate antigens and antibodies.
A test to separate plasma or urine proteins. The method can be used to identify monoclonal (abnormal) immunoglobulins. These proteins are important diagnostic features of myeloma and some lymphomas.
Technique combining protein electrophoresis and double immunodiffusion. See ELECTROPHORESIS. Proteins are separated by agarose-gel electrophoresis, then specific anti-sera are placed in a trough cut parallel to the protein track. Proteins and antibodies are allowed to diffuse through the gel. Proteins branch out in all directions from a common center. Antibodies diffuse perpendicularly from the trough, resulting in a distinct elliptical precipitin arc.
See Immunofixation electrophoresis.
A two step procedure which first involves the electrophoretic separation of proteins, followed by the linear diffusion of antibodies into the electrophoretic gel from a trough which extends through the length of the gel adjacent to the electrophoretic path.
Pertaining to investigation of substances and their identifications by a method combining mobility in an electric field and utilizing an antigen-antibody reaction. It refers to a combination technique used to elevate proteins in a mixture, or the characteristics of a specific protein.
Immunoelectrophoresis (IES) is the electrophoresis of a determined antigen mixture in an agarose gel that allows the separation of different proteins along the gel slide, and then the lateral diffusion in the gel of an immune serum or a monoclonal antibody. If some antibodies are specific to one of the antigens, the prepicitation of the antigen-antibody complexes revealed a precipitacion arc evidenciable by the eye over a dark background, or by Coomassie blue staining.
