detection of cell characteristics by use of fluorescent dyes, lasers and monoclonal antibodies in a flow by system. Cells are tagged with the dye and directed in single file through a laser beam. Fluorescent intensity and number of cells exhibiting fluorescence is determined. Cells may be separated according to the intensity of their fluorescence.

The analysis and sorting of cells that have been reacted with fluorescent probes. The sorting process is based on detection of fluorescence emitted by single cells within droplets.

The technique of measuring optical properties of individual cells, or particles in general, in a flow stream rapidly passing one-at-a-time in front of a laser beam with detectors measuring fluorescence and light scatter. A flow cytometer can measure these parameters at rates of thousands of cells per minute.

A procedure used to evaluate the risk of recurrence of certain cancers by measuring the amount of DNA in cells.

A method of sperm sorting used for sex selection. X and Y chromosome bearing sperm are stained with different flourescent dyes and then can be sorted by colour.

A method for the separation of chromosomes by detecting the light-absorbing or fluorescing properties of cells or subcellular fractions (i.e., chromosomes) passing in a narrow stream through a laser beam.

Analysis and sorting of cells or subcellular fractions that have been reacted with fluorescent probes by detecting their light-absorbing or fluorescing properties passing in a narrow stream through a laser beam. An absorbance or fluorescence profile of the sample is produced.

a powerful tool to study loss of HLA class I expression in cell suspensions from fresh human cervical tumors

a suitable method for the evaluation of physiological or hormonal influences on cell cycle kinetics of human anagen hair bulbs in vivo

This is a laboratory method where blood is placed into a machine that separates blood cells by size, shape, density and epitope (antennae) on their surface. The immunophenotype assay and the NK assay are tested by using this machine. Each cell in the machine passes a laser eye and the reflected light is analyzed by the machine. Each type of cell reflects light differently. An NK ( CD 56+) cell gives a different reflection than a B cell (CD 19+).

Test that measures DNA content in tumors. Understanding Breast Cancer

(flo sie-TAH-meh-tree) Diagnostic test for cancerous cells that uses the arrangement and amount of DNA in a cell to distinguish benign cells from malignant ones, or to monitor the effect of anticancer treatment

a technique used to measure the optical properties of cells in a flow stream rapidly passing one-at-a-time in front of a laser beam with detectors measureing fluorescence and light scatter

method of analysing cells according to coloration with labelled antibodies[ back

Laser based method of determining cell cycle characteristics. -- Excellent in depth description of flow cytomety from the University of Florida

Method used to measure nuclear DNA quantity in order to determine ploidy status.

A laboratory technique in which cell numbers and characteristics, such as size, shape or density, are measured using fluorescent dyes.

Automated analysis of cells or subcellular components by detection of the fluorescence or light-scatter of sample fractions passing in narrow-stream droplets through a laser beam.

Analysis of biological material by detection of the light- absorbing or fluorescing properties of cells or subcellular fractions (i.e., chromosomes) passing in a narrow stream through a laser beam. An absorbance or fluorescence profile of the sample is produced. Automated sorting devices, used to fractionate samples, sort successive droplets of the analyzed stream into different fractions depending on the fluorescence emitted by each droplet.

A procedure that measures the amount of DNA in cells.

a procedure that uses a laser-powered instrument to measure the amount of DNA in cells. It may be used to evaluate risk of the recurrence of some cancers. An abnormal amount of DNA may indicate a greater risk of recurrence.

the process of identifying cells using a battery of antibodies for specific cell markers

(FC), is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerized instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorized first by size and then computer analyzed to sort the mixture of cellular elements into cell type by size. In addition, monoclonal antibodies to specific cell surface markers are conjugated to fluorescent dyes and each cell displaying appropriate fluorescent light emission is counted. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal conjugates are utilized, even when the size of the cell is identical to other subset species.

A method of measuring the number of cells in a sample, the percentage of live cells in a sample, and certain characteristics of cells, such as size, shape, and the presence of tumor markers on the cell surface. The cells are stained with a light-sensitive dye, placed in a fluid, and passed in a stream before a laser or other type of light. The measurements are based on how the light-sensitive dye reacts to the light.

A test of tumor tissue to see how fast the tumor cells are reproducing and whether the tumor cells contain a normal or abnormal amount of DNA. This test is used to help predict how aggressive a cancer is likely to be. ( See also ploidy, DNA, S-phase fraction).

Flow cytometry is a method used to count cells (especially blood cells), using a glass chamber of known volume.

a technique for rapid automatic separation of suspensions of living cells into defined sub-populations.

A technology utilizing a laser beam to analyze and count individual cells in a rapidly flowing fluid stream. It is possible to study DNA content, different types of blood cells and tumor cells.

A method of measuring physical and chemical attributes of cells or other biological particles by sensors, as they move by, one after the other, homogeneously suspended in fluid. This rapid (about 10,000 particles per second) analytic method allows identification of important cell types, such as malignant cells, T cells and B cells. This process also may utilize immunologic binding methods to further identify particular cell groups.

See Fluorescent activated cell sorting.

Analysis of cell populations in suspension according to each individual cell's expression of selected surface markers.

Flow cytometry is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus.