Restriction fragment length polymorphism. A genotyping technique based on measuring the number and length of specific DNA fragments that are cut using specific restriction enzymes. The RFLP technique used to genotype M. tuberculosis is based on the IS 6110 insertion sequence.
restriction fragment length polymorphism. variation in the lengths of restriction fragments of DNA. These fragments are obtained from different individuals due to genetic polymorphism of the restriction sites in the chromosomes. It is also known as DNA fingerprinting.
See Genetic variations glossary Polymorphic sequences that result in RFLPs are used as markers on both physical maps and genetic linkage maps. RFLPs are usually caused by mutation at a cutting site.