Proteins isolated from bacteria that cut DNA at specific sequences of As, Cs, Gs, and Ts.
Class of enzymes that cut DNA at specific locations identified by the sequence of the nucleotides. At the site of the cut other pieces of DNA, sometimes sharing the same recognition sequence, can be inserted next to the original location of the cut.
enzymes used to isolate gene sized DNA fragments. The enzymes react chemically with a specific base sequence in the DNA molecule and break the molecule at that point. Restriction enzymes are made by bacteria.
Enzymes that can cut strands of DNA at specific base sequences.
catalyze the opening of a DNA molecule at a "restriction" point; many leave dangling ends of DNA molecules at the point where the DNA has been opened.
A common name for restriction endonucleases
These are enzymes (endonucleases, more specifically) which recognize a specific, short sequence of DNA and cut the DNA at that point. Different restriction enzymes recognize and cut different sequences. There are hundreds of different restriction enzymes available commercially. Many restriction enzymes leave "sticky ends" when they cut, which are available to bind with other "sticky ends" left by the same enzymer. Restriction enzymes are a vital tool in genetics, since they allow cutting (and pasting) of DNA.
level: Comprehensive (3) [ order by level] Biological molecules often used in biotechnology to cut pieces of DNA. While they are very useful to molecular biologists, their natural function in bacteria is to protect the cell from viral attacks: these enzymes recognize the viral DNA or RNA as foreign and cut it to pieces, thereby destroying the virus. The bacterium's own DNA is spared, a cunning example of natural selection.
An enzyme (normally derived from bacteria) that cuts strands of DNA, at particular points along its length into shorter fragments.
enzymes that cut DNA molecules at specific nucleotide sequences depending on the particular enzyme used. DNA molecules from different organisms may be cut up into pieces of unique sizes which may be seen by separating these pieces by gel electrophoresis. Enzyme recognition sites are usually 4 to 6 base pairs in length. Generally, the shorter the recognition sequence, the greater the number of fragments generated.
An enzyme that recognizes and cleaves DNA at specific sequences. They are useful to scientists for analyzing gene structure and mutations.
Enzymes that recognize a specific sequence of double-stranded DNA and cut the DNA at that site. Restriction enzymes are often referred to as molecular scissors.
A series of enzymes that attach to DNA molecules at speciÞc nucleotide sequences and cut both strands of DNA at those sites. A bacterial enzyme that cuts DNA at a specific recognition sequence. This is a bacterial defense against viral DNA and plasmid DNA and is now used as an important tool in biotechnology.
Biological scissors (enzymes) that recognize and cut a specific DNA sequence.
proteins that cleave DNA at positions determined by the specific order or sequence of DNA bases
Enzymes cutting and recognizing specific short nucleotide segments within a DNA sequence. The molecular biologist uses restriction enzymes as "scissors" for cutting DNA. Within the DNA sequence they recognize specific nucleotide sequences, so called palindromes, where they "cut". There are a vast number of restriction enzymes recognizing and cutting different sequences.
Enzymes produced by bacteria that break 'foreign' DNA into segments, acting at specific base sequences to do this. Different restriction endonucleases have different base-sequence sites at which they act. Many of them produce sticky ends on the products of their activity. These enzymes are sometimes known as "gene shears" and are represented in diagrams as scissors.
Enzymes that recognize specific DNA sequences and cleave them to produce DNA fragments of varying length. The ability to specifically cleave DNA sequences at known sites underlies many of the advances made over the last several decades in molecular biology.
a protein that can recognize specific short sequences of (usually) unmethylated DNA and cuts DNA at those sites
DNA-cutting enzymes that recognize and bind to specific short sections of DNA sequence.
Enzymes that recognize specific regions of a long DNA molecule and cut it at those points.