Definitions for "Restriction endonuclease"
enzymes produced in various bacterial species that cleave DNA molecules at specific nucleotide sequences. Used in recombinant DNA technology. Named for their role in restricting the host range of bacteriophage.
Enzyme that breaks nucleic acid (usually DNA) at only one specific sequence of nucleotides.
A class of enzymes that cut DNA after recognizing a specific sequence. The three types of restriction endonuclease are: I. Where the cut occurs within a random sequence at sites 1kbp from the recognition sequence, and has both restriction and methylation activities. II: Cuts within, or near a short, usually palindromic recognition sequence. A separate enzyme methylates the same recognition sequence. III: Cuts 24-26bp downstream from a short, asymmetrical recognition sequence, requires ATP and has both restriction and methylation activities. Type II enzymes are the class used for most molecular biology applications.