The joining of two linear nucleic acid molecules by the formation of phospho-diester bonds. In cloning experiments, a restriction fragment is often ligated to a linearized vector molecule using T4 DNA ligase.
Joining of DNA fragments to produce a single DNA molecule. Ligases are enzymes which perform this reaction. Fragments of plant DNA are ligated into bacterial plasmids during the cloning of probes for use for RFLP analysis.
The process of splicing two pieces of DNA together. In practice, a pool of DNA fragments are treated with ligase (see "Ligase") in the presence of ATP, and all possible splicing products are produced, including circularised forms and end-to-end ligation of 2, 3 or more pieces. Usually, only some of these products are useful, and the investigator must have some way of selecting the desirable ones.
In vitro reaction used to make the covalent phosphodiester linkage between two double stranded DNA ends. The reaction requires the presence of a phosphate group on the free 5' end and a -OH group on the free 3' end of the strands to be joined. The reaction is catalysed by the enzyme DNA ligase.
The surgical process of tying off an anatomical channel, often a blood vessel, preventing the flow of blood. Also used with a tubal ligation, tying off the fallopian tubes preventing the exchange of ova to the uterus.