A technique utilized to localize nucleic acid segments complementary to specific labeled probes. To localize specific DNA sequences, specimens are treated so as to denature DNAs and to remove adhering RNAs and proteins. The DNA segments of interest are then detected via hybridization with labeled nucleic acid probes. --Click Here For Details
A variation of the DNA/RNA hybridization procedure in which the denatured DNA is in place in the cell and is then challenged with RNA or DNA extracted from another source. (See also fluorescence in situ hybridization).
A method where single stranded DNA or RNA probes are used to locate a gene or mRNA molecule within a cell or tissue. Unlike standard hybridization procedures, in situ hybridization allows one to pinpoint the location of the gene or mRNA of interest.
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g. plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH). This is distinct from immunohistochemistry, which localizes proteins in tissue sections. DNA ISH can be used to determine the structure of chromosomes.