Any chemical method that uses an antibody labeled with a fluorescent dye. Test is called DIRECT if a specific antibody or anti-serum is conjugated with a fluorochrome and used as a specific fluorescent stain. Test is called INDIRECT if the fluorochrome is attached to an anti-globulin and a tissue constituent is stained using an unlabeled specific antibody and the labeled anti-globulin, which binds the unlabeled antibody.
Technique in which one or other component of an immunological reaction is made fluorescent by coupling with a fluorochrome so that the occurrence of the reaction can be detected as fluorescing complexes.
(immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissue
A technique used to identify particular antigens microscopically in tissues or on cells by the binding of a fluorescent antibody conjugate.
serologic procedure using antibody labelled with a fluorescent dye that allows visible detection of sites of reaction with antigen
The emission of visible light by a compound that has been irradiated with ultraviolet light. For example, a fluorescent compound (i.e., a fluorescein) can be attached to an antibody. Bacterial, viral, or other antigens that react with the antibody can then be observed by illuminating the sample with ultraviolet light.
a technique for showing up specific molecules or structures in cells or tissues to be viewed under a microscope. Because antibodies can be made to specifically bind to any molecule, they are used in this technique. A fluorescent molecule (which glows when illuminated with UV light) is stuck onto the "beacon" end of a specific antibody which can then make certain bits of a cells or tissues glow (see a picture)
technique allowing the visualisation of a specific protein in cells or tissue sections (prepared from a biopsy) by binding a specific antibody conjugated to a fluorescent substrate.
A method of locating antigens in tissue, or of identifying an antigen by the fluorescence resulting when the antigenic material is exposed to a specific antibody coupled with a fluorescent dye. The antigen-antibody combination then is visible (luminous) using ultraviolet light. This technique is used in the diagnosis of syphilis, toxoplasmosis (a serious disease which may lead to blindness and brain defects in the congenital form, as well as myocarditis and other conditions), and other diseases.
Immunofluorescence is the labeling of antibodies or antigens with fluorescent dyes. This technique is sometimes used to make viral plaques more readily visible to the human eye. Immunofluorescent labelled tissue sections are studied using a fluorescence microscope or by confocal microscopy.