is an analytical-chemistry-based tool for analyzing mixtures of chemical compounds. It is used to determine the amount of a chemical compound within a mixture of other chemicals, for example the exact amount of active pharmaceutical ingredient in a drug. The sample is dissolved in a solvent (like water or alcohol) and a detector measures response changes between the solvent itself, and the solvent and sample when passing through it. The electrical response is digitized and sent to a data system. Click here for more information.
HPLC. An efficient form of column chromatography that pumps a liquid solution of the sample at very high pressure through a column packed with a stationary phase made of very tiny particles. The high pressure pumps required make HPLC an expensive technique.
a sensitive technique for separating and analysing mixtures. It is a form of liquid chromatography in which the sample is forced through the chromatography column under pressure by high-pressure pumps. HPLC is useful for separating compounds that are dissolved in solution and results in an excellent separation in a relatively short time.