a set of cDNA clones prepared from the mRNA isolated from a particular type of tissue
A library composed of cDNAs, not necessarily representing all mRNAs.
A gene library composed of cDNA inserts synthesized from mRNA using reverse transcriptase.
A set of DNA fragments prepared from the total mRNA obtained from a selected cell, tissue or organism.
a collection of cDNA's, each of which has been inserted in a DNA vector (e.g. a circular DNA plasmid) and replicated in a bacterium such as E. coli. The bacteria maintain a ready pool of the cDNA's and can be cultured to make copies of the library for many experiments. A population of bacteria containing a single inserted cDNA is called a clone. To extract a cDNA from the library, the insert from the corresponding clone can be amplified using PCR primed off the vector sequences connected to the ends of the cDNA.
A collection of DNA sequences that code for genes. The sequences are generated in the laboratory from mRNA sequences. See also: messenger RNA
Library composed of complementary copies of cellular mRNAs. Represents all of the expressed DNA in a cell
A collection of cloned DNA molecules that are complementary to the entire set of mRNA molecules obtained from a cell, organism, or tissue.
A collection of DNA sequences generated from mRNA sequences. This type of library contains only protein-coding DNA (genes) and does not include any non-coding DNA.
In molecular biology, a cDNA library refers to a complete, or nearly complete, set of all the m RNAs contained within a cell or organism. Because working with mRNA is difficult (as mRNA is unstable and is easily degraded by RNases which can be found even on the skin), researchers use an enzyme called reverse transcriptase which will produce a DNA copy of each mRNA strand. Referred to as c DNA these reverse transcribed mRNAs are collectively known as the library.
