A technique that uses x-ray film to detect labeled molecules after hybridization of a labeled DNA or RNA probe to DNA or RNA within tissue specimens.
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned bacterial or cultured eukaryotic cells.
A technique for gene mapping involving hybridization of a labelled sample of a cloned gene to a large DNA molecule (usually a chromosome), often within a cell.
The pairing of a labeled probe to its complementary sequence within intact, banded chromosomes.
A technique utilized to localize nucleic acid segments complementary to specific labeled probes. To localize specific DNA sequences, specimens are treated so as to denature DNAs and to remove adhering RNAs and proteins. The DNA segments of interest are then detected via hybridization with labeled nucleic acid probes. --Click Here For Details
In situ hybridization (ISH) is a laboratory technique that uses chemically or radioactively-labelled pieces of DNA to detect specific genes or chromosomes in cells. (cf FISH)
A variation of the DNA/RNA hybridization procedure in which the denatured DNA is in place in the cell and is then challenged with RNA or DNA extracted from another source. (See also fluorescence in situ hybridization).
a method used to detect and locate specific DNA or RNA sequences.
Use of a nucleic acid probe to detect and identify specific complementary sequences of DNA in chromosomes or RNA in bacteria, eukaryotic cells, and tissue.
The base pairing of a sequence of DNA to metaphase chromosomes on a microscope slide.
A method where single stranded DNA or RNA probes are used to locate a gene or mRNA molecule within a cell or tissue. Unlike standard hybridization procedures, in situ hybridization allows one to pinpoint the location of the gene or mRNA of interest.
Hybridization with a DNA probe carried out directly on a chromosome preparation or histological section.
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g. plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH). This is distinct from immunohistochemistry, which localizes proteins in tissue sections. DNA ISH can be used to determine the structure of chromosomes.