A cloning vector that contains the necessary regulatory sequences to allow transcription and translation of a cloned gene or genes.
A modified plasmid or virus that carries a gene or cDNA into a suitable host cell and there directs synthesis of the encoded protein. Some expression vectors are designed for screening DNA libraries for a gene of interest ( Figures 7-21 and 20-9); others, for producing large amounts of a protein from its cloned gene ( Figures 7-36 and 7-37).
virus or plasmid that harbours a DNA sequence constructed in the aim of expressing a specific protein into a host cell.
A virus or plasmid that carries a DNA sequence into a suitable host cell and there directs the synthesis of a specific protein.
A vector that has been designed to express cloned genes in a particular cell type.
In recombinant DNA work, a type of vector that contains promoters and other regulatory sequences that enable a protein-coding DNA inserted into the vector to be transcribed and translated if introduced into the appropriate cells.
DNA vector, such as a plasmid, that carries a DNA sequence that includes the adjacent sequences for its expression into mRNA and protein in a host cell.
Plasmid or virus vector that carries into a cell a DNA sequence that directs the synthesis of a protein.
a DNA molecule comprising a gene that is expressed in a host cell
a DNA molecule, linear or circular, that comprises a DNA segment encoding a foreign DNA of interest operably linked to additional segments that provide for its transcription
a nucleic acid construct, generated recombinantly or synthetically, bearing a series of specified nucleic acid elements that enable transcription of a particular gene in a host cell
a nucleic acid construct, typically generated recombinantly or synthetically, with a series of specified nucleic acid elements which permit transcription of a particular nucleic acid in a host cell
a plasmid derived construction designed to enable the expression of a gene carried on the vector
a relatively small DNA molecule that can be used to carry a specific gene into a target cell
a replicable DNA construct in which a DNA sequence encoding a protein or peptide as given herein is operably linked to suitable control sequences capable of effecting the expression of the DNA sequence in a suitable host
a vector which (due to the presence of appropriate transcriptional and/or translational control sequences) is capable of expressing a DNA (or cDNA) molecule which has been cloned into the vector and of thereby producing a polypeptide or protein
A vector which is designed to allow expression (transcription and translation) of the inserted section of DNA. The vector carries a promoter (normally inducible) on one side of the cloning site, and a transcription terminator on the other side. These must be recognisable by the intended host cell. The vector may also carry a ribosome binding sequence (for bacterial expression) and a start codon, depending on the nature of the inserted DNA. Some expression vectors produce fusion proteins.
A way of delivering foreign genes to a host, creating a recombinant organism that will express the desired protein.
A type of cloning vector that enables the foreign gene it carries to be expressed as polypeptide in the recipient host cell.
A cloning vector that is engineered to allow the expression of protein from a cDNA. The expression vector provides appropriate promoter and restriction sites that allow insertion of cDNA.
Typically a small, circular piece of DNA that is transformed (inserted) into a particular expression host for the purpose of producing the protein coded for by the DNA.
The vector into which the gene of interest has been cloned.
A cloning vector that contains regulatory sequences that promote transcription and translation of a cloned gene.
is a cloning vector designed so that a coding sequence inserted at a particular site will be transcribed and translated into protein.
A cloning vector designed so that a foreign gene inserted into the vector will be expressed in the host organism.
An expression vector is a relatively small DNA molecule that is used to introduce and express a specific gene into a target cell. Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular transcription and translation machinery.