Randomly selected, partial cDNA sequence; represents it's corresponding mRNA. dbEST is a large database of ESTs at GenBank, NCBI.
A unique stretch of DNA within a coding region of a gene that is useful for identifying full-length genes and serves as a landmark for mapping.
Partial gene sequence data of a cDNA clone which provides a sequence tag for a gene. In order to achieve a very high throughput, these sequences are usually only subjected to a single-pass of sequencing so the error rate in these sequences can be high, perhaps approaching 5%.
A clone from a cDNA library for which a partial sequence has been generated.
A partial sequence of an expressed gene. EST sequence is obtained by high- throughput methods and is useful in the mapping of genomes.
A unique segment of cDNA with a base sequence identical to at least part of the coding region of a gene, generally used as landmark for mapping.
A partial sequence of a clone, randomly selected from a cDNA library and used to identify genes expressed in a particular tissue. ESTs are used extensively in projects to map the human genome.
a sequence from a transcribed mRNA for a possibly unknown gene. Individual EST's are neither especially informative nor highly accurate; however, they are easy to collect in large numbers. A database of EST's can be helpful for picking protein-coding sequences out of a long stretch of DNA, or for providing a larger context for very short sequences, such as the small pieces of protein sequence obtainable by mass spectrometry. EST's are compiled by the National Center for Biotechnology Information in a database called dbEST.
tags of expressed sequence that can be used as landmarks along the human genome and to provide clues about the location of new and similar genes.
a short strand of DNA (approximately 200 base pairs long) which is part of a cDNA. Because an EST is usually unique to a particular cDNA, and because cDNAs correspond to a particular gene in the genome, ESTs can be used to help identify unknown genes and to map their position in the genome.
A short DNA sequence from a coding region that is used to identify a gene.
A known cDNA sequence of several hundred nucleotides, which forms part of a gene and is derived from RNA. The RNA usually codes for a protein or protein fragment of unknown function.
(EST) A gene fragment of 300 to 400 nucleotides that has been sequenced from a cDNA clone.
An expressed sequence tag or EST is a short sub-sequence of a transcribed spliced nucleotide sequence (either protein-coding or not). They are intended as a way to identify gene transcripts, and are instrumental in gene discovery and gene sequence determination. The identification of ESTs has proceeded rapidly, with approximately 37 million ESTs now available in public databases (e.g.