Means two different things to eukaryotic and prokaryotic geneticists! First used for the transformation of prokaryotic cells by protein-free DNA or RNA from viruses. Also refers to the process of genetic transformation in eukaryotic cells.
introduction of genes directly into a cell by chemical or physical processes that make the cell membrane temporarily permeable to foreign DNA.
The introduction of DNA into a cell or organism using recombinant DNA technology.
Introduction of a foreign DNA molecule into a eucaryotic cell; usually followed by expression of one or more genes in the newly introduced DNA.
new DNA being introduced to a cell, integrated, and expressed, as in viral DNA, recombinant DNA, or cancerous DNA.
The introduction of purified phage DNA into a bacterial cell by transformation or electroporation.
an efficient means for generating RNA-transduced DCs and for stimulating antigen-specific T cell responses
the transfer of DNA to the cell of a higher organism, such as an animal or a crop plant
A general term to describe the introduction of recombinant or vector DNA into host cells.
The transfer of new genetic material into cells.
Alteration of the genome of a cell by direct introduction of DNA, a small portion of which becomes covalently associated with the host cell DNA.
The process of placing foreign DNA into mammalian cells.
Experimental introduction of foreign DNA into cells in culture, usually followed by expression of genes in the introduced DNA.
Uptake, incorporation, and expression of recombinant DNA
Introduction of foreign DNA into a eukaryotic host cell, followed by its integration into the chromosomal DNA.
permanently changing a cell using viral DNA.
The introduction of foreign DNA into a host cell. See also: cloning vector, gene therapy
A method by which experimental DNA may be put into a cultured mammalian cell. Such experiments are usually performed using cloned DNA containing coding sequences and control regions (promoters, etc) in order to test whether the DNA will be expressed. Since the cloned DNA may have been extensively modified (for example, protein binding sites on the promoter may have been altered or removed), this procedure is often used to test whether a particular modification affects the function of a gene.
Transfer of DNA (usually of a gene) into a cultured cell where it can be expressed.
The transfer of DNA to an eukaryotic cell.
A method of introduction of purified viral DNA into cells.
The process by which exogenous DNA in solution is introduced into cultured cells.
introduction of a foreign gene (DNA) into a cell's genome.
the alteration of the genetic code within a cell by the addition of exogenous genetic sequences
Introduction of a foreign DNA molecule into a eucaryotic cell and subsequent expression of the genes of the new DNA.
The transfer, for the purposes of genomic integration, of naked, foreign DNA into cells in culture. The traditional microbiological usage of this term implied that the DNA being transferred was derived from a virus. The definition as stated here is that which is in use to describe the general transfer of DNA irrespective of its source. (See also "transformation".)
The introduction of DNA into a recipient cell and its subsequent integration into the recipient cell's chromosomal DNA.
The successful virus-infection of cells following their inoculation with viral nucleic acid. ( 10)
Transfection is the introduction of foreign DNA into eukaryotic or prokaryotic cells, such as animal or bacterial cells. Transfection typically involves opening transient "holes" or gates in cells to allow the entry of extracellular molecules, typically supercoiled plasmid DNA, but also si RNA, among others. Cells that have been manipulated to accept foreign DNA (cells with "holes") are called "competent cells".