A procedure for manufacturing proteins by manipulation of DNA segments. Used to produce large quantities of enzymes for use in enzyme replacement therapy.
Procedures used to join together DNA segments in a cell-free system (an environment outside a cell or organism) to make recombinant DNA. The techniques involved in the construction, study and use of recombinant DNA molecules.
Procedure used to join together DNA segments in a test tube. Under appropriate conditions, a recombinant DNA molecule can enter a cell and replicate there, either autonomously or after it has become integrated into a chromosome. The technique of cutting DNA from one organism and inserting it into the DNA of another, invented in 1972, created the field of genetic engineering.
See Genetic Engineering.
Modern techniques in molecular biology to manipulate an organism's genes by introducing, eliminating, or changing genes.
Procedures used to join together DNA segments in vitro.
"The ability to chop up DNA, the stuff of which genes are made, and move the pieces, [which] permits the direct examination of the human genome," and the identification of the genetic components of a wide variety of disorders [Holtzman (1989), p. 1]. Recombinant DNA technology is also used to develop diagnostic screens and tests, as well as drugs and biologics for treating diseases with genetic components. See OHRP IRB Guidebook Chapter 5, Section H, "Human Genetic Research."
Techniques used to take apart and recombine a cell's genetic information, and being investigated for use in melanoma vaccines.
A set of techniques for manipulating DNA, including: the identification and cloning of genes; the study of the expression of cloned genes; and the production of large quantities of gene product.
Is the technology that has allowed the transfer of already existing genes from one organism to another with special carriers or vectors, such as bacteria and viruses.
The techniques by which scientists insert, delete, or change stretches of DNA in living organisms.
The techniques and tools employed to produce recombinant DNA.
the technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism
techniques involving modifications of an organism by incorporation of DNA fragments from other organisms using techniques of molecular biology.
the deliberate insertion of genes into a DNA molecule using the techniques of modern molecular biology
The technique of isolating genes from one organism and purifying and reproducing them in another organism; sometimes called genetic engineering.
Procedures used to join together DNA segments in a cell-free system (e.g. in a test tube outside living cells or organisms). Under appropriate conditions, a recombinant DNA molecule can be introduced into a cell and copy itself (replicate), either as an independent entity (autonomously) or as an integral part of a cellular chromosome.
Techniques for cutting apart and splicing together peices of DNA, frequently from differenct organisms.
The process of excising segments of DNA from one species of organism and inserting them into the DNA of another species.
the technique by which genetic material from one organism is inserted into a foreign cell in order to mass produce the protein encoded by the inserted genes.
A series of techniques in which DNA fragments are linked to self-replicating forms of DNA to create recombinant DNA molecules. These molecules in turn are replicated in a host cell to create clones of the inserted segments.
A series of procedures used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed (recombined) from segments from 2 or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, autonomously (on its own) or after it has become integrated into a chromosome.
A set of techniques which enable one to manipulate DNA. One of the main techniques is DNA cloning (because it produces an unlimited number of copies of a particular DNA segment), and the result is sometimes called a DNA clone or gene clone (if the segment is a gene), or simply a clone. An organism manipulated using recombinant DNA techniques is called a genetically modified organism (GMO). Among other things, recombinant DNA technology involves: - identifying genes; - cloning genes; - studying the expression of cloned genes; and - producing large quantities of the gene product.
The application of genetic tools (restriction endonucleases, plasmids, and transformation) to the production of specific proteins by biological "factories" such as bacteria.
A powerful technology based on the ability to manipulate and propagate DNA segments (e.g. genes). Recombinant DNA technology has numerous applications. For instance, it can be used in the field of vaccine development to produce abundant amounts of antigens for use in vaccines or to genetically modifiy pathogens to produce attenuated vaccines.
Procedure used to join together DNA segments in a cell-free system (an environment outside a cell or organism). Under appropriate conditions, a recombinant DNA molecule can enter a cell and replicate there, either autonomously or after it has become integrated into a cellular chromosome.
a broad term referring to molecular cloning as well as techniques for making recombinant DNA or using it for specific purposes.
A range of biochemical techniques that enable the precise cutting and joining of DNA molecules (genes) at will in a test-tube and their subsequent introduction into organisms.
Techniques for cutting apart, splicing together, and producing pieces of DNA from different sources.
Artificial rearrangement of DNA; segments of DNA from one organism can be incorporated into the genetic makeup of another organism. Using these techniques, researchers can study the characteristics and actions of specific genes. Many modern genetic research methods are based on recombinant DNA technology.