Definitions for "Isoelectric focusing"
An electrophoresis technique based on the movement of proteins through a stable pH gradient established using carrier ampholytes. Isoelectric focusing is the first dimension of 2-D electrophoresis.
The electrophoretic migration of proteins in a pH gradient to the pH at which they have no net charge (the isoelectric point). Proteins which diffuse away from the isoelectric pH become charged again and are electrophoresed back to their isoelectric pH (thus isoelectric focussing).
Technique for separating molecules by gel electrophoresis in a pH gradient subjected to an electric field. A protein migrates to the pH at which its overall net charge is zero.