Denaturing gradient gel electrophoresis. Method to separate DNA or proteins by altering the pH inside a gel. As the molecules migrate in the electric field, they encounter denaturing conditions, which slow down their mobility. Different molecules respond differently to the denaturation condition and therefore respond at a different severity of denaturant (resulting in a loss of mobility at a different point in the gel).

denaturing gradient gel electrophoresis. It is used to separate DNA fragments of the same length but with different sequences. Separation is based on the decreased electrophoretic mobility of a partially melted double-stranded DNA molecule in polyacrylamide gel containing a linear gradient of DNA denaturants. See -- TGGE.

Denaturing Gradient Gel Electrophoresis. Molecular technique to study the structure of the microbial community and its dynamics. Double stranded DNA with the same length but a different basepair sequence is loaded on a denaturing gel. After separation of the strands, a band pattern is obtained in which in theory each band corresponds to one type of bacterium. Genes used in this technique are the 16S rRNA gene (a gene that is present in all bacteria and that enables to distinguish one bacterial species from another) and catabolic genes.